Volume 1, Issue 1 (Winter-Spring 2017)                   Mod Med Lab J 2017, 1(1): 23-28 | Back to browse issues page

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Yeganeh Montakhab H, Dousti M, Ahmadi Rezaie H B. Assessment of the Relative Level of TNF-α Gene Expression in RAW264.7 Cells. Mod Med Lab J. 2017; 1 (1) :23-28
URL: http://modernmedlab.com/article-1-39-en.html
Abstract:   (4647 Views)

Background and Objective: Recent studies have shown that not only the quantity, but also the quality of dietary fatty acids can affect lipid parameters. Elaidic acid is a major hydrogenated by-product of vegetable oil play a role in the development of cardiovascular disease, especially atherosclerosis, through changing lipid profile and increase in inflammation and inflammatory factors. On the other hand, the role of TNF-α is evident in inflammatory pathways and inflammation outcomes, which are involved in the initiation of atherosclerosis. Therefore, in the current study the effect of elaidic acid on TNF-α gene expression, was investigated.  

Method: RAW264.7 cells were treated with 100 and 200 μM of elaidic acid for 12 h. Then, expression of TNF-α gene was assessed after RNA extraction and cDNA synthesis. 

Results:  TNF-α gene expression in RAW264.7 macrophage cell line showed no significant difference for both concentrations compared to the control. 

Conclusion: These results indicated that elaidic acid affect the development and acceleration of atherosclerosis in other ways than through TNF-α nuclear receptor. 

Full-Text [PDF 339 kb]   (1371 Downloads)    
Type of Study: Original | Subject: Biochemistry

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