Volume 1, Issue 1 (Spring 2017)                   Mod Med Lab J 2017, 1(1): 23-28 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yeganeh Montakhab H, Dousti M, Ahmadi Rezaie H B. Assessment of the Relative Level of TNF-α Gene Expression in RAW264.7 Cells. Mod Med Lab J. 2017; 1 (1) :23-28
URL: http://modernmedlab.com/article-1-39-en.html
Abstract:   (2879 Views)

Background and Objective: Recent studies have shown that not only the quantity, but also the quality of dietary fatty acids can affect lipid parameters. Elaidic acid is a major hydrogenated by-product of vegetable oil play a role in the development of cardiovascular disease, especially atherosclerosis, through changing lipid profile and increase in inflammation and inflammatory factors. On the other hand, the role of TNF-α is evident in inflammatory pathways and inflammation outcomes, which are involved in the initiation of atherosclerosis. Therefore, in the current study the effect of elaidic acid on TNF-α gene expression, was investigated.  

Method: RAW264.7 cells were treated with 100 and 200 μM of elaidic acid for 12 h. Then, expression of TNF-α gene was assessed after RNA extraction and cDNA synthesis. 

Results:  TNF-α gene expression in RAW264.7 macrophage cell line showed no significant difference for both concentrations compared to the control. 

Conclusion: These results indicated that elaidic acid affect the development and acceleration of atherosclerosis in other ways than through TNF-α nuclear receptor. 

Full-Text [PDF 338 kb]   (835 Downloads)    
Type of Study: Original | Subject: Biochemistry

References
1. Mbewu A, Mbanya JC. Cardiovascular Disease. In: Jamison DT, Feachem RG, Makgoba MW, Bos ER, Baingana FK, Hofman KJ, et al., editors. Disease and Mortality in Sub-Saharan Africa. 2nd ed. Washington (DC): World Bank; 2006.
2. Charo IF, Taub R. Anti-inflammatory therapeutics for the treatment of atherosclerosis. Nat Rev Drug Discov 2011;10(5):365-76. [DOI:10.1038/nrd3444] [PMID] [PMCID]
3. Libby P, Ridker PM, Hansson GK; Leducq Transatlantic Network on Atherothrombosis. Inflammation in atherosclerosis: from pathophysiology to practice. J Am Coll Cardiol 2009 1;54(23):2129-38.
4. Rivera MF, Lee JY, Aneja M, Goswami V, Liu L, Velsko IM, et al. Polymicrobial infection with major periodontal pathogens induced periodontal disease and aortic atherosclerosis in hyperlipidemic ApoE(null) mice. PLoS One. 2013;8(2):e57178. [DOI:10.1371/journal.pone.0057178] [PMID] [PMCID]
5. Tabaka M. The effects of polyunsaturated fatty acids on coronary disease. JAAPA 2008;21(5):39-40, 42-4. [DOI:10.1097/01720610-200805000-00012] [PMID]
6. Cox RA, Garcia-Palmieri MR. Cholesterol, Triglycerides, and Associated Lipoproteins. In: Walker HK, Hall WD, Hurst JW, editors. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd ed. Boston: Butterworths;1990.
7. Schaefer EJ, Gleason JA, Dansinger ML. Dietary fructose and glucose differentially affect lipid and glucose homeostasis. J Nutr 2009;139(6):1257S-62S. [DOI:10.3945/jn.108.098186] [PMID] [PMCID]
8. Ciccone MM, Cortese F, Gesualdo M, Carbonara S, Zito A, Ricci G, et al. Dietary intake of carotenoids and their antioxidant and anti-inflammatory effects in cardiovascular care. Mediators Inflamm 2013;2013:782137. [DOI:10.1155/2013/782137] [PMID] [PMCID]
9. Sampath H, Ntambi JM. Polyunsaturated fatty acid regulation of genes of lipid metabolism. Annu Rev Nutr 2005;25:317-40. [DOI:10.1146/annurev.nutr.25.051804.101917] [PMID]
10. Benatar JR, Gladding P, White HD, Zeng I, Stewart RA. Trans-fatty acids in New Zealand patients with coronary artery disease. Eur J Cardiovasc Prev Rehabil 2011;18(4):615-20. [DOI:10.1177/1741826710389415] [PMID]
11. Dhaka V, Gulia N, Ahlawat KS, Khatkar BS. Trans fats-sources, health risks and alternative approach - A review. J Food Sci Technol 2011;48(5):534-41. [DOI:10.1007/s13197-010-0225-8] [PMID] [PMCID]
12. Boden G. Obesity. insulin resistance and free fatty acids. Curr Opin Endocrinol Diabetes Obes 2011;18(2):139-43. [DOI:10.1097/MED.0b013e3283444b09] [PMID] [PMCID]
13. Soto-Vaca A, Losso JN, McDonough K, Finley JW. Differential effect of 14 free fatty acids in the expression of inflammation markers on human arterial coronary cells. J Agric Food Chem 2013;61(42):10074-9. [DOI:10.1021/jf402966r] [PMID]
14. Akira S, Hirano T, Taga T, Kishimoto T. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J 1990;4(11):2860-7. [DOI:10.1096/fasebj.4.11.2199284] [PMID]
15. Barbier O, Torra IP, Duguay Y, Blanquart C, Fruchart JC, Glineur C, et al. Pleiotropic actions of peroxisome proliferator-activated receptors in lipid metabolism and atherosclerosis. Arterioscler Thromb Vasc Biol 2002;22(5):717-26. [DOI:10.1161/01.ATV.0000015598.86369.04] [PMID]
16. Upadhyay RK. Emerging risk biomarkers in cardiovascular diseases and disorders. J Lipids 2015;2015:971453. [DOI:10.1155/2015/971453] [PMID] [PMCID]
17. Siddiqui RA, Harvey KA, Ruzmetov N, Miller SJ, Zaloga GP. n-3 fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death. Br J Nutr 2009;102(12):1811-9. [DOI:10.1017/S0007114509992030] [PMID]
18. Siri-Tarino PW, Sun Q, Hu FB, Krauss RM. Saturated fatty acids and risk of coronary heart disease: modulation by replacement nutrients. Curr Atheroscler Rep 2010;12(6):384-90 . [DOI:10.1007/s11883-010-0131-6] [PMID] [PMCID]
19. Mozaffarian D, Pischon T, Hankinson SE, Rifai N, Joshipura K, Willett WC, et al. Dietary intake of trans fatty acids and systemic inflammation in women. Am J Clin Nutr 2004;79(4):606-12. [DOI:10.1093/ajcn/79.4.606] [PMID] [PMCID]
20. Peluso I, Raguzzini A, Villano DV, Cesqui E, Toti E, Catasta G, et al. High fat meal increase of IL-17 is prevented by ingestion of fruit juice drink in healthy overweight subjects. Curr Pharm Des 2012;18(1):85-90. [DOI:10.2174/138161212798919020] [PMID]
21. Bickel M. The role of interleukin-8 in inflammation and mechanisms of regulation. J Periodontol 1993;64(5 Suppl):456-60. [PMID]
22. Frohnert BI, Jacobs DR, Jr., Steinberger J, Moran A, Steffen LM, Sinaiko AR. Relation between serum free fatty acids and adiposity, insulin resistance, and cardiovascular risk factors from adolescence to adulthood. Diabetes 2013;62 (9):3163-9. [DOI:10.2337/db12-1122] [PMID] [PMCID]
23. Schepers A, Eefting D, Bonta PI, Grimbergen JM, de Vries MR, van Weel V, et al. Anti-MCP-1 gene therapy inhibits vascular smooth muscle cells proliferation and attenuates vein graft thickening both in vitro and in vivo. Arterioscler Thromb Vasc Biol 2006;26(9):2063-9. [DOI:10.1161/01.ATV.0000235694.69719.e2] [PMID]
24. Egashira K. Molecular mechanisms mediating inflammation in vascular disease: special reference to monocyte chemoattractant protein-1. Hypertension 2003;41(3 Pt 2):834-41. [DOI:10.1161/01.HYP.0000051642.65283.36] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2017 All Rights Reserved | Modern Medical Laboratory Journal