Modern Medical Laboratory Journal

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Cells that make up the body's tissues are usually three-dimensional architecture, the threedimensional culture system enables cells to create natural and in vivo interactions which is an ideal environment for 3D (Three-dimensional) cell growth and issues such as exchange of similar food exchanges inside Capillary in living tissue. In tissue engineering discussion, cell scaffolding is highly important and is used for tissue implantation, which will eventually dissolve or demolish after applying to the body. Bone formation can occur via two separate pathways, within the cartilaginous and within the membrane. The vascular endothelial growth factor is a key regulator of angiogenesis. The combination of osteoactivity is a major parameter in the engineering structures and the function of the bones and osteoblasts is very important in maintaining and repairing bone in the laboratory conditions. Also, in this study, we investigated transcription factors, epigenetic reforms, miRNAs, and Sox family in tissue engineering. Between transcription factors, TLX's core receiver is essential for maintaining neural stem cells and NCSs reviver. Epigenetic reforms maybe a major factor that links genetic and environmental factors to the risk of osteoporosis. Micro-RNAs regulate bone and chondrogenic differentiation by targeting important transcription factors and relative pathways during skeleton maturation. The members of the Sox family interact in a wide range of cellular tissues and thus create a variety of effects on cellular metabolism.

Keywords: Tissue engineering, Cell scaffolding, vascular endothelialgrowth factor

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