Volume 1, Issue 2 (Summer-Fall 2018)                   Mod Med Lab J 2018, 1(2): 77-83 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Afshari F, Seraj H, Sadat Hashemi Z, Timajchi M, Ensiyeh O, Ladan G, et al . The Cytotoxic Effects of Eggplant Peel Extract on Human Gastric Adenocarcinoma Cells and Normal Cells. Mod Med Lab J 2018; 1 (2) :77-83
URL: http://modernmedlab.com/article-1-42-en.html
Abstract:   (8921 Views)

Background and objective: Gastric cancer is the fourth common and the second deadliest cancer worldwide, which the major causes of its prevalence are genetic factors and the lifestyle, such as eating fast foods, physical inactivity, environmental pollution, and other natural factors.

Method: This research was performed in human and animal cell bank of Iranian Biological Resource Center, and cytotoxic effects of the eggplant peel extract was investigated on human gastric cancer cells and normal cells. During this experiment, all quality control procedures were performed on cell lines, including bacteria, fungi, yeasts, molds, mycoplasma, as well as HIV-I, HBV, HCV, EBV viruses, and the results of negative tests were reported. MTT test was used to determine the cytotoxicity of this substance and the IC50 value.

Resutls: The results indicated that the toxic effect of eggplant extract was more on the cancer cells compared to the normal cells and it is notable that, the death rate of the cancer cells in three concentrations showed a significant difference compared to the normal cells. 

Conclusion: From the results of this study, it is recommended that further investigations be conducted on eggplant extract as an anticancer nutrient.

Full-Text [PDF 318 kb]   (7558 Downloads)    

References
1. Zhao Y, Feng F, Zhou YN. Stem cells in gastric cancer. World J Gastroenterol 2015;21(1):112-23. [DOI:10.3748/wjg.v21.i1.112] [PMID] [PMCID]
2. Hurt RT, Kulisek C, Buchanan LA, McClave SA. The obesity epidemic: challenges, health initiatives, and implications for gastroenterologists. Gastroenterol Hepatol (N Y) 2010;6(12):780-92.
3. Rates SM. Plants as source of drugs. Toxicon 2001;39(5):603-13. [DOI:10.1016/S0041-0101(00)00154-9]
4. Noda Y, Kneyuki T, Igarashi K, Mori A, Packer L. Antioxidant activity of nasunin, an anthocyanin in eggplant peels. Toxicology 2000;148(2-3):119-23. [DOI:10.1016/S0300-483X(00)00202-X]
5. Luthria DL, Mukhopadhyay S. Influence of sample preparation on assay of phenolic acids from eggplant. J Agric Food Chem 2006;54(1):41-7. [DOI:10.1021/jf0522457] [PMID]
6. Salerno L, Modica MN, Pittala V, Romeo G, Siracusa MA, Di Giacomo C, et al. Antioxidant activity and phenolic content of microwave-assisted Solanum melongena extracts. ScientificWorldJournal 2014;2014:719486. [DOI:10.1155/2014/719486] [PMID] [PMCID]
7. Bayan L, Koulivand PH, Gorji A. Garlic: a review of potential therapeutic effects. Avicenna J Phytomed 2014;4(1):1-14. [PMID] [PMCID]
8. Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 2001;12(7):404- 21. [DOI:10.1016/S0955-2863(01)00155-3]
9. Vujicic M, Nikolic I, Kontogianni VG, Saksida T, Charisiadis P, Orescanin-Dusic Z, et al. Methanolic extract of Origanum vulgare ameliorates type 1 diabetes through antioxidant, anti-inflammatory and anti-apoptotic activity. Br J Nutr 2015;113(5):770-82. [DOI:10.1017/S0007114514004048] [PMID]
10. Weisburger JH, Chung FL. Mechanisms of chronic disease causation by nutritional factors and tobacco products and their prevention by tea polyphenols. Food Chem Toxicol 2002;40(8):1145-54. [DOI:10.1016/S0278-6915(02)00044-3]
11. Zou T, Wang D, Guo H, Zhu Y, Luo X, Liu F, et al. Optimization of microwave-assisted extraction of anthocyanins from mulberry and identification of anthocyanins in extract using HPLC-ESI-MS. J Food Sci 2012;77(1):C46-50. [DOI:10.1111/j.1750-3841.2011.02447.x] [PMID]
12. Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. A microwell cytotoxicity assay using Artemia salina (brine shrimp). Planta Med 1993;59(3):250-2. [DOI:10.1055/s-2006-959661] [PMID]
13. Azuma K, Ohyama A, Ippoushi K, Ichiyanagi T, Takeuchi A, Saito T, et al. Structures and antioxidant activity of anthocyanins in many accessions of eggplant and its related species. J Agric Food Chem 2008;56(21):10154-9. [DOI:10.1021/jf801322m] [PMID]
14. Smit HF, Woerdenbag HJ, Singh RH, Meulenbeld GJ, Labadie RP, Zwaving JH. Ayurvedic herbal drugs with possible cytostatic activity. J Ethnopharmacol 1995;47(2):75-84. [DOI:10.1016/0378-8741(95)01255-C]
15. Ames BN. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 1983;221(4617):1256-64. [DOI:10.1126/science.6351251]
16. Suresh R, Ali S, Ahmad A, Philip PA, Sarkar FH. The Role of Cancer Stem Cells in Recurrent and Drug-Resistant Lung Cancer. Adv Exp Med Biol 2016;890:57-74. [DOI:10.1007/978-3-319-24932-2_4] [PMID]
17. Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci 2005;78(5):431-41. [DOI:10.1016/j.lfs.2005.09.012] [PMID]
18. Wiseman M. The second World Cancer Research Fund/American Institute for Cancer Research expert report. Food, nutrition, physical activity, and the prevention of cancer: a global perspective. Proc Nutr Soc 2008;67(3):253-6. [DOI:10.1017/S002966510800712X] [PMID]
19. Prasain JK, Barnes S. Metabolism and bioavailability of flavonoids in chemoprevention: current analytical strategies and future prospectus. Mol Pharm 2007;4(6):846-64. [DOI:10.1021/mp700116u] [PMID]
20. Vance TM, Su J, Fontham ET, Koo SI, Chun OK. Dietary antioxidants and prostate cancer: a review. Nutr Cancer 2013;65(6):793-801. [DOI:10.1080/01635581.2013.806672] [PMID] [PMCID]
21. Kocic B, Kitic D, Brankovic S. Dietary flavonoid intake and colorectal cancer risk: evidence from human population studies. J BUON 2013;18(1):34-43. [PMID]
22. Neuhouser ML. Dietary flavonoids and cancer risk: evidence from human population studies. Nutr Cancer 2004;50(1):1-7. [DOI:10.1207/s15327914nc5001_1] [PMID]
23. Middleton E, Jr., Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 2000;52(4):673-751. [PMID]
24. Borek C. Dietary antioxidants and human cancer. Integr Cancer Ther 2004;3(4):333-41. [DOI:10.1177/1534735404270578] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Modern Medical Laboratory Journal