Volume 1, Issue 1 (Winter-Spring 2018)                   Mod Med Lab J 2018, 1(1): 36-41 | Back to browse issues page

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Azimi S, Basei Salehi M, Bahador N. Isolation and Identification of Streptomyces ramulosus from Soil and Determination of Antimicrobial Property of its Pigment. Mod Med Lab J 2018; 1 (1) :36-41
URL: http://modernmedlab.com/article-1-41-en.html
Isolation and Identification of Streptomyces ramulosus from Soil and Determination of Antimicrobial Property of its Pigment
Saba Azimi , Majid Basei Salehi , Nima Bahador
Abstract:   (7687 Views)

Background and objective: Pigment production by microorganisms is important due to more rapid growth, higher efficiency, easier extraction, and antimicrobial effects compared to other methods. The aim of the present study is to isolate and identify antibiotic pigment-producing actinomycetes from the soil of the Persian Gulf and to evaluate the antibiotic activity of their pigments in the second half of 2014. Given that pathogenic bacteria increasingly become more resistant to common antibiotics, there is a need for new antibiotics.

Methods: A total of 80 soil samples were collected from different areas and after preparing different dilutions (10-1-10-7), each sample was cultured on nutrient agar medium, specific Starch Casein Agar (SCA), and the International Streptomyces Project 2 (ISP2) media. Then, the actinomycete colonies were selected based on pigment production; pigments were extracted using sediment extraction with ethyl acetate; and the antibacterial property of their pigments were evaluated. The structure of colored extract compounds was identified by Thin layer chromatography (TLC). Molecular identification of the microorganisms was performed by 16S rRNA PCR.

Results: The highest antibiotic effect of the pigments was observed in pathogenic bacterium S. aureus and Bacillus cereus, respectively. The lowest antimicrobial activity of the pigments was observed in Escherichia coli bacterium. All the studied fungi were resistant to the studied pigments.

Conclusion: Since natural pigments at an appropriate dose are healthier than the chemical ones, using them is beneficial in various industries, such as antibiotics production. Also, by determining the structure of pigments and eliminating their toxic groups, they can be used in different industries.

Keywords: Actinomycete, Pigment, Antimicrobial property
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Type of Study: Original Research Article | Subject: Medical Biochemistry
References
1. Chaudhary HS, Yadav J, Shrivastava AR, Singh S, Singh AK, Gopalan N. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). J Adv Pharm Technol Res. 2013;4(2):118-23. [DOI:10.4103/2231-4040.111528] [PMID] [PMCID]
2. Dsouza M, Taylor MW, Turner SJ, Aislabie J. Genome-based comparative analyses of Antarctic and temperate species of Paenibacillus. PLoS One 2014;9(10):e108009. [DOI:10.1371/journal.pone.0108009] [PMID] [PMCID]
3. Ribes JA, Vanover-Sams CL, Baker DJ. Zygomycetes in human disease. Clin Microbiol Rev 2000;13(2):236-301. [DOI:10.1128/CMR.13.2.236-301.2000] [PMID] [PMCID]
4. Valour F, Senechal A, Dupieux C, Karsenty J, Lustig S, Breton P, et al. Actinomycosis: etiology, clinical features, diagnosis, treatment, and management. Infect Drug Resist 2014;7:183-97. [PMID] [PMCID]
5. Woznicka W. The Significance of Variation of Some Antibiotic Actinomycetes for the Taxonomy of Microorganisms of This Genus. Arch Immunol Ther Exp (Warsz) 1964;12:37-54.
6. Jose PA, Jebakumar SR. Phylogenetic diversity of actinomycetes cultured from coastal multipond solar saltern in Tuticorin, India. Aquatic biosystems 2012;8(1):23. [DOI:10.1186/2046-9063-8-23] [PMID] [PMCID]
7. Krewski D, Yokel RA, Nieboer E, Borchelt D, Cohen J, Harry J, et al. Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide. J Toxicol Environ Health B Crit Rev 2007;10 Suppl 1:1-269. [DOI:10.1080/10937400701597766] [PMID] [PMCID]
8. Mizukami H, Konoshima M, Tabata M. Variation in pigment production in Lithospermum erythrorhizon callus cultures. Phytochemistry 1978;17(1):95-7. [DOI:10.1016/S0031-9422(00)89687-9]
9. Gonzalez Holgado G, Castro Rodriguez J, Canedo Hernandez LM, Diaz M, Fernandez-Abalos JM, Trujillano I, et al. Radamycin, a novel thiopeptide produced by streptomyces sp. RSP9. I. Taxonomy, fermentation, isolation and biological activities. J Antibiot (Tokyo) 2002;55(4):383-90. [DOI:10.7164/antibiotics.55.383]
10. Maleki H, Mashinchian O. Characterization of Streptomyces Isolates with UV, FTIR Spectroscopy and HPLC Analyses. Bioimpacts 2011;1(1):47-52. [PMID] [PMCID]
11. Chau R, Kalaitzis JA, Wood SA, Neilan BA. Diversity and biosynthetic otential of culturable microbes associated with toxic marine animals. Mar Drugs 2013;11(8):2695-712. [DOI:10.3390/md11082695] [PMID] [PMCID]
12. Gebreyohannes G, Moges F, Sahile S, Raja N. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Biomed 2013;3(6):426-35. [DOI:10.1016/S2221-1691(13)60092-1]
13. Hussain MS, Fareed S, Ansari S, Rahman MA, Ahmad IZ, Saeed M. Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci 2012;4(1):10-20. [DOI:10.4103/0975-7406.92725] [PMID] [PMCID]
14. Bokulich NA, Bamforth CW. The microbiology of malting and brewing. Microbiol Mol Biol Rev 2013;77(2):157-72. [DOI:10.1128/MMBR.00060-12] [PMID] [PMCID]
15. McMurray, DN. Mycobacteria and Nocardia. In: Baron S, editor. Medical Microbiology. 4th edition. Galveston (TX): University of Texas Medical Branch at Galveston; 1996.
16. Ghannoum MA, Rice LB. Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin Microbiol Rev 1999;12(4):501-17. [PMID] [PMCID]
17. Morris-Jones R, Gomez BL, Diez S, Uran M, Morris-Jones SD, Casadevall A, et al. Synthesis of melanin pigment by Candida albicans in vitro and during infection. Infect immun 2005;73(9):6147-50. [DOI:10.1128/IAI.73.9.6147-6150.2005] [PMID] [PMCID]
18. Mayuran S, Radhakrishnan M, Balagurunathan R. Antimicrobial pigments from desert soil actinomycetes. In: proceeding of the National Symposium on "Recent Trends in Microbial Biotechnology". Sri Sankara Arts and Science College. 2006; p 35-51.
19. Sathi ZS, Sugimoto N, Khalil MI, Gafur M. Isolation of yellowish antibiotic pigment 4-hydroxy nitrobenzene from a strain of Streptomyces. Pak J Biol Sci 2002;52:201-3.
20. Wang L, Lei J, Xiang W, Jiang S, Sun J, Yang Z. Identification and characterization of purple pigment-producing actinomycete Strain. Chin J Appl Environ Biol 2009;15(1):139-42.
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