Volume 4, Issue 2 (Summer-Fall 2021)                   Mod Med Lab J 2021, 4(2): 36-39 | Back to browse issues page


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Benhamza N, Sebbar E, Naili A, Choukri M. Comparison of two automated methods for the measurement of glycated hemoglobin HBA1C: ADAMS A1c (ARKRAY) versus Capillarys 2 Flex Piercing® (SEBIA). Mod Med Lab J. 2021; 4 (2) :36-39
URL: http://modernmedlab.com/article-1-105-en.html
Abstract:   (129 Views)
Introduction: The aim of this work is to present the results of a comparative study between the determination of glycated hemoglobin (HBA1C) on the ADAMS A1c® (ARKRAY) and Capillarys 2 Flex Piercing® (SEBIA).
Materials and methods: 310 venous blood samples were randomly selected from routine HBA1C tests. The HBA1C assay was performed on ADAMS A1c® (ARKRAY) and Capillarys 2 Flex Piercing® (SEBIA) during the same day. The data obtained were analyzed by the statistical software MedCalc Version 15.1.0.
Results: The results obtained show a good correlation between the 2 methods: the equation of the Passing-Bablok line is of type Y (Capillarys 2 Flex Piercing®) = -0.550 + 1.119 X (ADAMS A1c) the 95% confidence interval of this slope is -0.6467 to - 0.4414 with r = 0.982 and p <0.0001. The Bland-Altman plot shows that the average bias between the two methods is in the order of 0.3 and that the difference between the Capillarys and HPLC measurements of Hba1c is in the range of +1.96 to -1.96 and the Deming regression equation Y (Capillarys 2 Flex Piercing®) = -0.3388 + 1.0911 X (ADAMS A1c).
Conclusion: Our study shows a good agreement of HBA1C results between the Capillarys 2 Flex Piercing® (SEBIA) and ADAMS A1c® (ARKRAY). Laboratory work requires professionals to take into account variations in results when changing methods in their routine work by comparing their results on the various methods.
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Type of Study: Brief report | Subject: Laboratory Methods

References
1. Gillery P. A history of HbA1c through Clinical Chemistry and Laboratory Medicine. Clin Chem Lab Med. 2013;51(1):65–74. [DOI:10.1515/cclm-2012-0548]
2. Chemsi H, Kamal N. Vérification de la méthode de dosage de l’HbA1c par HPLC au laboratoire de biochimie. Rev francoph lab. 2020;2020(523):22–7. [DOI:10.1016/S1773-035X(20)30195-7]
3. Lamri MA, Himeur MA, Idir KA, Chikouche A. L’intérêt de garder la même technique de dosage de l’hémoglobine glyqué (HbA1c) pour le suivi du diabète en Algérie. Ann Endocrinol (Paris) . 2018;79(4):471. [DOI:10.1016/j.ando.2018.06.908]
4. Maleska A, Hirtz C, Casteleyn E, Villard O, Ducos J, Avignon A, et al. Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system. Bioanalysis. 2017;9(5):427–34. [DOI:10.4155/bio-2016-0278]
5. Assia Yasmine B, Farah N. Etude comparative des techniques du dosage de l’hémoglobine glyquée. 2020. [Article]
6. Urrechaga E. High-resolution HbA(1c) separation and hemoglobinopathy detection with capillary electrophoresis. Am J Clin Pathol. 2012;138(3):448–56. [DOI:10.1309/AJCPVYW9QZ9EVFXI]
7. Bouchrata SM. DETECTION DES VARIANTS DE L’HEMOGLOBINE LORS DU DOSAGE DE L’HBA1C PAR CLHP A ECHANGE CATIONIQUE. EXPERIENCE DU LABORATOIRE DE BIOCHIMIE – TOXICOLOGIE DE L’HMIMV DE RABAT. 2018. [Article]
8. GOSSIN G, GUILLERM M, PING J, FARGES G. La qualité au sein des Laboratoires de Biologie Médicale selon l’ISO 15189 v2012. [Article]
9. Arvier M, Chevailler A. Harmonisation du système de management de la qualité au sein d’un laboratoire de biologie médicale hospitalo-universitaire. Rev francoph lab. 2018;2018(506):22–5. [DOI:10.1016/S1773-035X(18)30317-4]

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