Volume 1, Issue 2 (Summer 2017)                   Mod Med Lab J 2017, 1(2): 42-49 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dortaj H, Anvari M, Yadegari M, Hosseini Sharifabad M, Abbasi Sarcheshmeh A. Stereological Survey of the Effect of Vitamin C on Neonatal Rat Kidney Tissue Treated With Acrylamide. Mod Med Lab J. 2017; 1 (2) :42-49
URL: http://modernmedlab.com/article-1-44-en.html
Abstract:   (2160 Views)

Background and Objectives: Acrylamide (ACR) is a known carcinogenic chemical agent found in some foods at considerably high concentration. The aim of this study was to investigate the protective effects of vitamin C as an antioxidant on kidney tissue in rats treated with ACR.

Methods: Twenty female Wistar rats (200-220 grams) were divided into 4 groups (n=5) of control group, ACR group, vitamin C group and ACR+vitamin C group. Pregnant rats were orally administered 10 mg/kg ACR and/or 200 mg/kg vitamin C. Six infants at day 21 after birth were randomly selected and weighted and placed under deep anesthesia. Their right kidneys were processed and stained with hematoxylin and eosine and periodic acid staining and studied using stereological methods. Data was analyzed using one way ANOVA and LSD test and means difference were considered significant at P<0.05.

Results: Mean body weight, kidney weight, kidney volume, the volume of cortex and medulla, number of glomeruli and thickness of medulla significantly decreased in ACR group compared to the controls (P<0.001). The thickness of cortex also decreased in ACR group compared to the control group (P<0.05). In vitamin C group, body weight, kidney volume and number of glomeruli increased compared to the control group (P<0.001). In vitamin C group, increased kidney weight, thickness of medulla, volume of cortex and glomeruli were observed compared to the control (P<0.05). In ACR+vitamin C group, this reduction was less significant compared to the ACR group.

Conclusion: Vitamin C as an antioxidant can protect the kidneys from ACR induced tissue damage.

Full-Text [PDF 573 kb]   (904 Downloads)    
Type of Study: Original | Subject: Histology

References
1. Khalil F, Aziem B. Effect of dietary acrylamide formed in potato crisps and toasted bread on rats. Egyptian Journal of Natural Toxins. 2005;2:57-70.
2. Lingnert H, Grivas S, Jägerstad M, Skog K, Törnqvist M, Åman P. Acrylamide in food: mechanisms of formation and influencing factors during heating of foods. Scandinavian Journal of Nutrition. 2002;46(4):159-72. [DOI:10.1080/110264802762225273]
3. Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. Journal of agricultural and food chemistry. 2002;50(17):4998-5006. [DOI:10.1021/jf020302f] [PMID]
4. Yerlikaya FH, Toker A, Yener Y. Effects of acrylamide treatment on oxidant and antioxidant levels in rats. Kafkas Universitesi Veteriner Fakultesi Dergisi. 2013;19(4):607-12. [DOI:10.9775/kvfd.2012.8518]
5. Kjuus H, Hansteen IL, Ryberg D, Goffeng LO, Øvrebø S, Skaug V. Chromosome aberrations in tunnel workers exposed to acrylamide and N-methylolacrylamide. Scandinavian journal of work, environment & health. 2005;31(4)300-6. [DOI:10.5271/sjweh.886] [PMID]
6. Schettgen T, Weiss T, Drexler H, Angerer J. A first approach to estimate the internal exposure to acrylamide in smoking and non-smoking adults from Germany. International journal of hygiene and environmental health. 2003;206(1):9-14. [DOI:10.1078/1438-4639-00195] [PMID]
7. Rydberg P, Eriksson S, Tareke E, Karlsson P, Ehrenberg L, Törnqvist M. Investigations of factors that influence the acrylamide content of heated foodstuffs. Journal of Agricultural and Food Chemistry. 2003;51(24):7012-8. [DOI:10.1021/jf034649+] [PMID]
8. Fuhr U, Boettcher MI, Kinzig-Schippers M, Weyer A, Jetter A, Lazar A, et al. Toxicokinetics of acrylamide in humans after ingestion of a defined dose in a test meal to improve risk assessment for acrylamide carcinogenicity. Cancer Epidemiology and Prevention Biomarkers. 2006;15(2):266-71. [DOI:10.1158/1055-9965.EPI-05-0647] [PMID]
9. Nixon B, Stanger S, Nixon B, Roman S. Chronic exposure to acrylamide induces DNA damage in male germ cells of mice. Toxicological Sciences. 2012;129(1):135-45. [DOI:10.1093/toxsci/kfs178] [PMID]
10. Schettgen T, Kütting B, Hornig M, Beckmann M, Weiss T, Drexler H, et al. Trans-placental exposure of neonates to acrylamide—a pilot study. International archives of occupational and environmental health. 2004;77(3):213-6. [DOI:10.1007/s00420-003-0496-8] [PMID]
11. Hogervorst JG, Schouten LJ, Konings EJ, Goldbohm RA, van den Brandt PA. Dietary acrylamide intake and the risk of renal cell, bladder, and prostate cancer. The American journal of clinical nutrition. 2008;87(5):1428-38. [PMID]
12. Boettcher MI, Bolt HM, Drexler H, Angerer J. Excretion of mercapturic acids of acrylamide and glycidamide in human urine after single oral administration of deuterium-labelled acrylamide. Archives of toxicology. 2006;80(2):55-61. [DOI:10.1007/s00204-005-0011-y] [PMID]
13. Maliakel DM, Kagiya TV, Nair CKK. Prevention of cisplatin-induced nephrotoxicity by glucosides of ascorbic acid and α-tocopherol. Experimental and Toxicologic Pathology. 2008;60(6):521-7. [DOI:10.1016/j.etp.2008.04.015] [PMID]
14. Kalender S, Kalender Y, Durak D, Ogutcu A, Uzunhisarcikli M, Cevrimli BS, et al. Methyl parathion induced nephrotoxicity in male rats and protective role of vitamins C and E. Pesticide biochemistry and physiology. 2007;88(2):213-8. [DOI:10.1016/j.pestbp.2006.11.007]
15. Hinchliffe S, Lynch M, Sargent P, Howard C, Velzen Dv. The effect of intrauterine growth retardation on the development of renal nephrons. BJOG: An International Journal of Obstetrics & Gynaecology. 1992;99(4):296-301. [DOI:10.1111/j.1471-0528.1992.tb13726.x]
16. Dezfoolian A, Panahi M, Feizi F. Stereological evaluation of renal glomeruli in offspring of diabetic female rats. Yakhteh Medical Journal. 2009;11(1):17-22.
17. Coulam CH, Bouley DM, Sommer FG. Measurement of renal volumes with contrast‐enhanced MRI. Journal Of Magnetic Resonance Imaging. 2002;15(2):174-9. [DOI:10.1002/jmri.10058] [PMID]
18. Howard V, Reed M. Unbiased stereology: three-dimensional measurement in microscopy: Garland Science; 2004.
19. Nyengaard J, Bendtsen T. Glomerular number and size in relation to age, kidney weight, and body surface in normal man. The Anatomical Record. 1992;232(2):194-201. [DOI:10.1002/ar.1092320205] [PMID]
20. Gage GJ, Kipke DR, Shain W. Whole animal perfusion fixation for rodents. JoVE (Journal of Visualized Experiments). 2012(65):e3564-e.
21. Sterio D. The unbiased estimation of number and sizes of arbitrary particles using the disector. Journal of microscopy. 1984;134(2):127-36. [DOI:10.1111/j.1365-2818.1984.tb02501.x] [PMID]
22. Bertram JF. Analyzing renal glomeruli with the new stereology. International review of cytology. 1995;161:111-72. [DOI:10.1016/S0074-7696(08)62497-3]
23. Skov K, Nyengaard JR, Korsgaard N, Mulvany MJ. Number and size of renal glomeruli in spontaneously hypertensive rats. Journal of hypertension. 1994;12(12):1373-6. [DOI:10.1097/00004872-199412000-00009] [PMID]
24. Organization WH. The world health report 2002: reducing risks, promoting healthy life: World Health Organization; 2002. 1. Eman M, Amany Y. Some studies on acrylamide intoxication in male albino rats. Egyptain Journal of Comparative Pathology and Clinical Pathology. 2008;21(4):222-45.
25. Mahmood SA, Amin KA, Salih SF. Effect of Acrylamide on Liver and Kidneys in Albino Wistar Rats. Int J Curr Microbiol App Sci. 2015;4(5):434-44.
26. Fennell TR, Friedman MA. Comparison of acrylamide metabolism in humans and rodents. Chemistry and Safety of Acrylamide in food: Springer; 2005;109-16. [DOI:10.1007/0-387-24980-X_9] [PMID]
27. Adeneye A, Olagunju J. Protective effect of oral Ascorbic Acid (Vitamin C) against Acetaminophen-induced renal injury in rats. African Journal of Biomedical Research. 2009;12(1):55-61.
28. Morsy FA. Protective effect of vitamin c and ginseng on experimental liver and kidney injuries induced by insecticide profenophos in male rats. Egypt J Hosp Med. 2003;10:34-51.

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2017 All Rights Reserved | Modern Medical Laboratory Journal