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Showing 3 results for Breast

Mohammad Reza Zinatizadeh, Zahra Masoumalinejad, Farzaneh Parnak,
Volume 2, Issue 1 (1-2019)
Abstract

Infectious agents cause 15-20% of cancers worldwide. The infectious agents and contamination may be caused by a local chronic and advanced local inflammatory response, or by tumorization. Mycoplasma contamination can interfere with biological agents and cause DNA damage which affects gene expression, and disrupts the control of cell cycle inspection and apoptotic responses. Mycoplasmas are widely distributed in nature; some mycoplasmas have the ability to penetrate into the cell and cause severe disease. Most mycoplasmas are known to infect the cell culture media, which is difficult to detect and contaminate. M. hyorhinis is one of the causes of Mycoplasma contamination in tissues samples from cancer patients. Mycoplasma is related with human cancers and several other human diseases. Several studies have shown that the potential role of M. hyorhinis includes esophageal, gastric, lung, breast, glioma, colon, and prostate cancers. The prevalence of M hyorhinis in various tissues leads to cancer progression. Therefore, it is necessary to pay more attention to this mycoplasma agent in order to control and understand its mechanism.
 
Mina Fartousi, Jamil Zargan, Shohreh Zare Karizi, Ms Sajedeh Zargan, Ashkan Haji Noor Mohammadi, Mohsen Mousavi, Hani Keshavarz Alikhani,
Volume 5, Issue 1 (1-2022)
Abstract

Objectives: Breast cancer is one of the most common types of cancer among women. One of these toxins that inhibits the growth of breast cancer cells in 2D cell culture and has significant anti-tumor effects is Dianthin-30. Therefore, in this manuscript, for the first time, the anti-cancer effect of Dianthin-30 toxin against breast cancer cells (MCF-7) in 3D culture has been studied.
Materials and Methods: In order to evaluate the anti-cancer effects and cytotoxicity of the toxin at concentrations of 1.25, 2.5, 5 and 10μg/ml, MTT methods were used and a Neutral red test was used to validate the results of this test. Nitric oxide, Catalase, GSH assays, cytochrome c, Caspase-3 and Comet assay tests were also used to determine the type of mortality in cancer cells.
Results: This toxin did not induce nitric oxide production, but at concentrations higher than 5μg/ml increased catalase production compared to the control. However, the level of GSH produced in all of the concentrations was significant compared to the control. In addition, Dianthin-30 increased cytochrome 30 and activation of caspase-3 in the above concentrations, but this effect was not significant compared to the control. The results of alkaline comet test also showed that the rate of induction of apoptosis by toxin was upward compared to the control.
Conclusion: The results of this study show that Dianthin-30 has anti-cancer effects and has caused death in breast cancer cells and this toxin probably induced apoptosis in cancer cells more than the non-mitochondrial pathway.

 
Dr Alireza Aleebrahim, Ms Farinaz Vafadar Esfahani,
Volume 6, Issue 1 (1-2023)
Abstract

Background :Breast cancer is a prevalent form of endocrine cancer that affects women globally, and its incidence and mortality rates are predicted to rise significantly in the coming years. As a result, breast cancer continues to pose a significant health issue and is a top priority for biomedical research. Methods: We used bioinformatics and reverse pharmacology techniques to identify herbal medicines that could be effective in treating breast cancer. To do this, we analyzed 121 genes from a dataset (GSE 42568) containing both cancer and normal samples. Through this analysis, we identified differentially expressed genes (DEGs) and then used the protein-protein interaction (PPI) network to identify 19 hub genes. To pinpoint hub genes, the widely-used bioinformatics tool, Search Tool for Retrieving Reciprocal Genes (STRING), was utilized. To conduct a more detailed analysis, subnetworks were identified using the MCODE algorithm. Results: The hub genes identified in our reaserch are involved in various functions, including positive regulation of cold-induced thermogenesis, patched binding, and the PPAR signaling pathway, as revealed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. We understood that the herbs Ginkgo biloba seeds, Polygoni Cuspidati Rhizoma Et Radix, Smilacis Glabrae Rhixoma, Capsici Fructus, Cyathulae Radix, Puerariae Flos, and Ardisiae Japonicae Herba can target hub genes such as PPARG, CCNB1, CAV1, CDH1, ADIPOQ, LEP, IGF1, LPL, DGAT2, ACSL1, and PCK1. Using nine identified ingredients, these herbs were identified as key in targeting hub genes. 
 

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