Volume 1, Issue 2 (Summer-Fall 2017)                   Mod Med Lab J 2017, 1(2): 50-59 | Back to browse issues page


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Sajjadi N, Mirnejad R, Sharif M, Piranfar V, Zaeifi D. Relevant Allelic Frequency of Gene Polymorphism and Genetic Predisposition of Human Papillomavirus in Patients with Cervical Cancer. Mod Med Lab J. 2017; 1 (2) :50-59
URL: http://modernmedlab.com/article-1-48-en.html
Abstract:   (8097 Views)

Backround: The present study investigated the correlation between p53 gene codon 72 polymorphism and 6 other genetic single nucleotide polymorphisms (SNPs) in patients with cervical cancer infected by HPV.

Methods: 450 patients with cervical cancer (280 Squamous cell carcinoma and 170 Adenocarcinoma) were followed at hospitals in Iran from Dec. 2014 to Apr. 2015. Moreover, 100 age/sex-matched were used as the control group. HPV was detected by LINEAR ARRAY® HPV Genotyping Test. Allelic frequency of 6 gene polymorphisms was detected by the amplification-refractory mutation system (ARMS).

Results: From 450 patients, 408 cases (90.66%) were positive for HPV. Four genotypes were observed as single infections (16, 18, 31, and 45). The most common genotypes were HPV-16 (73.52%), HPV-18 (23.28%), HPV-31 and 45 (3.17%), respectively. 306 samples were arginine-arginine homozygous (70.6% and 71.4% of adenocarcinoma and squamous cell carcinoma, respectively), 70 cases were arginine-proline heterozygous (17.6% of adenocarcinoma and 23.8% of squamous cell carcinoma), and 20 cases were as proline-proline homozygous (11.8% and 4.8% of adenocarcinoma and squamous cell carcinoma, respectively).

Conclusion: The prevalence of HPV was 84% and that was the estimation of the Global Burden among Iranian patients with cervical cancer (85% - 99%). There was no correlation between mutations in the p53 allele and the size/type of tumors, while we found a correlation between mutations in p53 alleles and age. Therefore, XRCC1 G399A SNP and TP53 G72C SNP were significantly correlated with the cervical cancer.

Abbreviations:

HPV: Human papillomavirus; SNP: Single nucleotide polymorphism; CDKN1A: cycling-dependent kinase inhibitor 1A; TP53: Tumor protein p53 (also known as p53); P53c72: p53 gene codon 72; ATM: Ataxia-telangiectasia mutated; HDM2: Human double minutes 2 (also known as MDM2); LIG4: DNA ligase IV; XRCC1: X-ray repair cross-complementing 1; XRCC3: X-ray repair cross-complementing 3; TGFB1: Transforming Growth Factor Beta 1; HWE’: Hardy-Weinberg equilibrium; FIGO stage: International Federation of Gynecology and Obstetrics tumor stage; 

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Type of Study: Original | Subject: Laboratory Methods

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