en روش های تشخیص آزمایشگاهی Laboratory Methods مقاله پژوهشی Original Research Article <div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background and Objective:</strong> It is conceivable that caffeine consumption would induce gonadal changes. The aim of this study is to assess the impact of embryonic caffeine exposure on rat testis and prostate.<br> <strong>Materials and Methods:</strong> Female rats were divided into (n=7): A control, only received drinking water. B and C groups received caffeine low dose (26 mg/kg) and high dose (45 mg/kg) respectively via drinking water during pregnancy and lactation. Structural changes in testis and prostate were studied by using stereological methods at 21, 60 and120 days of postnatal development.<br> <strong>Results:</strong> Our result showed decreases in body and testis weight of offspring of group C compared to other groups at all ages (P< 0.05). The Testis volume showed significant differences between the offspring of both experimental groups and control at days 21, 60 and 120 (P<0.05). There were significant differences in the number of sperm cells of offspring of experimental groups compared to the control group in different ages (P<0.05). The number of sertoli, spermatocyte and spermatid cells of offspring in group C showed a significant decrease compared with other groups at all days (P<0.01). The number of spermatogonia cells in group C offspring showed a significant decrease compared to the control group at different days (P<0.05). The mean Johnsen score decreased in offspring of group C compared to the control group (P<0.05).<br> <strong>Conclusion:</strong> Results showed that maternal caffeine consumption altered the structure of testis and prostate gland and spermatogenesis of offspring in adulthood</span></div> Caffeine, Testis, Prostate,Stereology,Rat offspring 46 59 http://modernmedlab.com/browse.php?a_code=A-10-59-1&slc_lang=en&sid=1 Mahboubeh Belali Kharaji foroozanbelali@gmail.com 10031947532846001447 10031947532846001447 No Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Maryam Yadegari yadegari_m@yahoo.com 10031947532846001448 10031947532846001448 Yes Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mohammad Hosseini Sharif abad mhosseini81@yahoo.com 10031947532846001449 10031947532846001449 No Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Morteza Anvari moanvari@yahoo.com 10031947532846001450 10031947532846001450 No Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Abolghasem Abbasi Sarcheshmeh abbasiyazdi@yahoo.com 10031947532846001451 10031947532846001451 No Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hengameh Dortaj hengameh.dourtaj@gmail.com 10031947532846001452 10031947532846001452 No Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran