fa میکروب شناسی بالینی Clinical Microbiology مقاله کوتاه پژوهشی Brief Original Article Pluripotent stem cells (PSCs) may be offered as an unlimited cell source for the hepatocyte generation. The generation of hepatocytes from stem cells in vitro would provide an alternative cell source for applications in drug discovery and cell transplantation. In this review, we discuss different approaches to generate pluripotent stem cell-derived hepatocytes, advantages, limitations for each method and finally, how three-dimensional (3D) strategy can improve the maturation of PSCs -derived hepatocytes.   Pluripotent stem cell, Hepatocyte differentiation, Tissue engineering, Organoids, Drug-screening 110 112 http://modernmedlab.com/browse.php?a_code=A-10-24-1&slc_lang=fa&sid=1 Ensieh Zahmatkesh 10031947532846001618 10031947532846001618 No Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Massoud Vosough masvos@royaninstitute.org 10031947532846001619 10031947532846001619 Yes Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran