Modern Medical Laboratory Journal
Mod Med Lab J
Medical Sciences
http://modernmedlab.com
1
admin
2371-770X
2371-770X
10.30699/mmlj17
en
jalali
1397
3
1
gregorian
2018
6
1
1
2
online
1
fulltext
en
Stereological Survey of the Effect of Vitamin C on Neonatal Rat Kidney Tissue Treated With Acrylamide
بافت شناسی
Histology
مقاله پژوهشی
Original Research Article
<p style="text-align: justify;"><strong><em>Background and Objectives</em></strong><strong>:</strong> Acrylamide (ACR) is a known carcinogenic chemical agent found in some foods at considerably high concentration. The aim of this study was to investigate the protective effects of vitamin C as an antioxidant on kidney tissue in rats treated with ACR.</p>
<p style="text-align: justify;"><strong><em>Methods</em></strong>: Twenty female Wistar rats (200-220 grams) were divided into 4 groups (n=5) of control group, ACR group, vitamin C group and ACR+vitamin C group. Pregnant rats were orally administered 10 mg/kg ACR and/or 200 mg/kg vitamin C. Six infants at day 21 after birth were randomly selected and weighted and placed under deep anesthesia. Their right kidneys were processed and stained with hematoxylin and eosine and periodic acid staining and studied using stereological methods. Data was analyzed using one way ANOVA and LSD test and means difference were considered significant at P<0.05.</p>
<p style="text-align: justify;"><strong><em>Results</em></strong><strong>:</strong> Mean body weight, kidney weight, kidney volume, the volume of cortex and medulla, number of glomeruli and thickness of medulla significantly decreased in ACR group compared to the controls (P<0.001). The thickness of cortex also decreased in ACR group compared to the control group (P<0.05). In vitamin C group, body weight, kidney volume and number of glomeruli increased compared to the control group (P<0.001). In vitamin C group, increased kidney weight, thickness of medulla, volume of cortex and glomeruli were observed compared to the control (P<0.05). In ACR+vitamin C group, this reduction was less significant compared to the ACR group.</p>
<p style="text-align: justify;"><strong><em>Conclusion: </em></strong>Vitamin C as an antioxidant can protect the kidneys from ACR induced tissue damage.</p>
Acrylamide, Ascorbic Acid, Kidney Injury, Rats, Stereology
42
49
http://modernmedlab.com/browse.php?a_code=A-10-33-1&slc_lang=en&sid=1
Hengameh
Dortaj
hengameh.dourtaj@gmail.com
10031947532846001573
10031947532846001573
No
Dept. of Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Morteza
Anvari
10031947532846001574
10031947532846001574
No
Reserch and Clinical Centre for infertility, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Maryam
Yadegari
yadegari_m@yahoo.com
10031947532846001575
10031947532846001575
Yes
Dept of Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mohammad
Hosseini Sharifabad
mhosseini@yahoo.com
10031947532846001576
10031947532846001576
No
Dept. of Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Abolghasem
Abbasi Sarcheshmeh
abbasiyazd@yahoo.com
10031947532846001577
10031947532846001577
No
Dept. of Anatomy and Cell Biology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran