en زیست شناسی پزشکی Medical Biology مقاله پژوهشی Original Research Article <div class="WordSection1"><span style="page:WordSection1"><span style="font-size:11pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:10.0pt">Objectives: Breast cancer is one of the most common types of cancer among women. One of these toxins that inhibits the growth of breast cancer cells in 2D cell culture and has significant anti-tumor effects is Dianthin-30. Therefore, in this manuscript, for the first time, the anti-cancer effect of Dianthin-30 toxin against breast cancer cells (MCF-7) in 3D culture has been studied.</span></span></span><br> <span style="font-size:11pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:10.0pt">Materials and Methods: In order to evaluate the anti-cancer effects and cytotoxicity of the toxin at concentrations of 1.25, 2.5, 5 and 10&mu;g/ml, MTT methods were used and a Neutral red test was used to validate the results of this test. Nitric oxide, Catalase, GSH assays, cytochrome c, Caspase-3 and Comet assay tests were also used to determine the type of mortality in cancer cells.</span></span></span><br> <span style="font-size:11pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:10.0pt">Results: This toxin did not induce nitric oxide production, but at concentrations higher than 5&mu;g/ml increased catalase production compared to the control. However, the level of GSH produced in all of the concentrations was significant compared to the control. In addition, Dianthin-30 increased cytochrome 30 and activation of caspase-3 in the above concentrations, but this effect was not significant compared to the control. The results of alkaline comet test also showed that the rate of induction of apoptosis by toxin was upward compared to the control.</span></span></span><br> <span style="font-size:11pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:10.0pt">Conclusion: The results of this study show that Dianthin-30 has anti-cancer effects and has caused death in breast cancer cells and this toxin probably induced apoptosis in cancer cells more than the non-mitochondrial pathway.</span></span></span></span></div> <div aria-label="Page Break" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="Page Break"></div><br> &nbsp; Breast cancer, Dianthins 30, Cytotoxicity, MCF-7 cell line, 3D culture 38 46 http://modernmedlab.com/browse.php?a_code=A-10-876-1&slc_lang=en&sid=1 Mina Fartousi mina.fartousi@gmail.com 10031947532846001863 10031947532846001863 No Department of Genetics, Faculty of Biological Sciences, Islamic Azad University ,Varamin -Pishva Branch, Varamin, Iran Jamil Zargan jzargan@yahoo.com 10031947532846001864 10031947532846001864 Yes Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran Shohreh Zare Karizi shohrehzare@yahoo.com 10031947532846001865 10031947532846001865 No Department of Genetics, Faculty of Biological Sciences, Islamic Azad University ,Varamin -Pishva Branch, Varamin, Iran Sajedeh Zargan zzargan.2013@gmail.com 10031947532846001866 10031947532846001866 No Department of Medical Radiation Engineering, Islamic Azad University, Central Tehran Branch, Tehran, Iran Ashkan Haji Noor Mohammadi ashkan.genetics@gmail.com 10031947532846001867 10031947532846001867 No Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran Mohsen Mousavi smohsenmousavi71@gmail.com 10031947532846001868 10031947532846001868 No Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran Hani Keshavarz Alikhani alikhani865@gmail.com 10031947532846001869 10031947532846001869 No Department of biology, Faculty of science, Razi University, Kermanshah, Iran