CAL.20C: More resistant SARS-CoV-2 variant to vaccines!

 | Post date: 2021/07/22 | 
A novel variant of concern (VOC) named CAL.20C (B.1.427/B.1.429), originally detected in California, carries spike glycoprotein mutations S13I in the signal peptide, W152C in the N-terminal domain (NTD), and L452R in the receptor-binding domain (RBD).
An international project which was done in the Department of Biochemistry at the University of Washington in Seattle indicates that the Epsilon variant is one of the most resistant SARS-CoV-2 variants to vaccines relying on an indirect and unusual neutralization-escape strategy. To learn more about the characteristics of this variant the researchers examined plasma from individuals vaccinated with a Wuhan-1 isolate-based mRNA vaccine or convalescent individuals. The results of this evaluation exhibited neutralizing titers, which were reduced 2-3.5 fold against the B.1.427/B.1.429 variant relative to wildtype pseudoviruses. The L452R mutation reduced neutralizing activity of 14 out of 34 RBD-specific monoclonal antibodies (mAbs). The S13I and W152C mutations resulted in total loss of neutralization for 10 out of 10 NTD-specific mAbs since the NTD antigenic supersite was remodeled by a shift of the signal peptide cleavage site and formation of a new disulphide bond, as revealed by mass spectrometry and structural studies.
Read more



CAPTCHA
View: 768 Time(s)   |   Print: 252 Time(s)   |   Email: 0 Time(s)   |   0 Comment(s)


© 2024 CC BY-NC 4.0 | Modern Medical Laboratory Journal