A small-molecule cocktail promotes cytoprotection and viability of human pluripotent and differentiated cells!

 | Post date: 2021/05/13 | 
The latest Nature publication proposes a small-molecule cocktail for the improvement of viability and differentiated progeny of Human pluripotent stem cells (hPSCs). hPSCs such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer an unmatched potential for disease modeling, drug
screening and development of cell therapies for intractable diseases. hPSCs are capable of extensive self-renewal yet remain highly sensitive to environmental perturbations in vitro, posing challenges to their therapeutic use. There is an urgent need to advance strategies that ensure safe and robust long-term growth and functional differentiation of these cells. In the latest Nature Methods publication, a small-molecule cocktail consists of chroman 1, emricasan, polyamines, and trans-ISRIB (CEPT) has been investigated for the enhancement of cell survival of genetically stable hPSCs by simultaneously blocking several stress mechanisms that otherwise compromise cell structure and function. CEPT provided strong improvements for several key applications in stem-cell research, including routine cell passaging, cryopreservation of pluripotent and differentiated cells, embryoid body (EB) and organoid formation, single-cell cloning, and genome editing. 
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