Volume 1, Issue 1 (Winter-Spring 2018)                   Mod Med Lab J 2018, 1(1): 12-16 | Back to browse issues page

‎ 10.30699/mmlj17-01-03

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Molecular Analysis of the Isolates of Acinetobacter baumannii isolated from Tehran Hospitals Using ERIC-PCR Method
Abbas Maleki , Jalil Vandyousefi , Zeinab Mirzaie , Sobhan Ghafourian , Hossein Kazemian , Noorkhoda Sadeghifard
Abstract:   (8564 Views)

Background and Objective: Acinetobacter is a genus of non-fermenting Gram-negative cocci or coccobacilli, which have low nutritional requirements for growth and can survive for a long time in adverse conditions, on dry surfaces, and also in aqueous environments. The importance of the members of Acinetobacter genus as pathogens involved in nosocomial infections, is increasing. Acinetobacter baumannii is the most common species involved in a broad spectrum of nosocomial infections, including pneumonia, bacteremia, surgical wound infections, urinary tract infections, and meningitis. In this study, enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) technique was used for analysis and molecular typing of Acinetobacter strains, which has high discrimination power compared to phenotypic markers. 

Methods: In the present study, a total of 40 A. baumanniies strains were isolated from patients hospitalized in Tehran hospitals. After identification and confirmation of the isolates by serotyping and biochemical tests, a single colony of each isolate was cultured on liquid LB medium, and after DNA extraction, PCR was performed. After electrophoresis of PCR product, gel images were stored electronically for analysis and comparison of the isolates. 

Results: In this study, 40 strains of A. baumannii were analyzed by ERIC-PCR method, of which 29 strains were typed into 10 groups and 11 other strains had no PCR bands or had a band that could not be assigned to any of the above groups.

Conclusion:  In this study, it was found that A. baumanniie strains could be typed using repetitive sequences. This extent of polymorphism shows that ERIC-PCR is a useful method for analysis of genetic variation of A. baumannii strains. High genetic variation of A. baumannii strains may be due to wide geographical distribution of this species in Iran 

Keywords: Acinetobacter baumannii, ERIC-PCR, Molecular Typing
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Type of Study: Original Research Article | Subject: Laboratory Methods
References
1. Visca P, Seifert H, Towner KJ. Acinetobacter infection--an emerging threat to human health. IUBMB life 2011;63(12):1048-54. [DOI:10.1002/iub.534] [PMID]
2. Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 2008;21(3):538-82. [DOI:10.1128/CMR.00058-07] [PMID] [PMCID]
3. Lin MF, Lan CY. Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside. World J Clin Cases 2014;2(12):787-814. [DOI:10.12998/wjcc.v2.i12.787] [PMID] [PMCID]
4. Viehman JA, Nguyen MH, Doi Y. Treatment options for carbapenem-resistant and extensively drug- resistant Acinetobacter baumannii infections. Drugs 2014;74(12):1315-33. [DOI:10.1007/s40265-014-0267-8] [PMID] [PMCID]
5. Richards AM, Abu Kwaik Y, Lamont RJ. Code blue: Acinetobacter baumannii, a nosocomial pathogen with a role in the oral cavity. Mol Oral Microbiol 2015;30(1):2-15. [DOI:10.1111/omi.12072] [PMID] [PMCID]
6. Roca I, Espinal P, Vila-Farres X, Vila J. The Acinetobacter baumannii Oxymoron: Commensal Hospital Dweller Turned Pan-Drug-Resistant Menace. Front Microbiol 2012;3:148. [DOI:10.3389/fmicb.2012.00148] [PMID] [PMCID]
7. Maleki MH, Sekawi Z, Soroush S, Azizi- Jalilian F, Asadollahi K, Mohammadi S, et al. Phenotypic and genotypic characteristics of tetracycline resistant Acinetobacter baumannii isolates from nosocomial infections at Tehran hospitals. Iran J Basic Med Sci 2014;17(1):21-6. [PMID] [PMCID]
8. Szejbach A, Mikucka A, Bogiel T, Gospodarek E. Usefulness of phenotypic and genotypic methods for metallo-beta-lactamases detection in carbapenem-resistant Acinetobacter baumannii strains. Med Sci Monit Basic Res 2013;19:32-6. [DOI:10.12659/MSMBR.883744] [PMID] [PMCID]
9. Howard A, O'Donoghue M, Feeney A, Sleator RD. Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence 2012;3(3):243-50. [DOI:10.4161/viru.19700] [PMID] [PMCID]
10. Martins HS, Bomfim MR, Franca RO, Farias LM, Carvalho MA, Serufo JC, et al. Resistance markers and genetic diversity in Acinetobacter baumannii strains recovered from nosocomial bloodstream infections. Int J Environ Res Public Health 2014;11(2):1465-78. [DOI:10.3390/ijerph110201465] [PMID] [PMCID]
11. Ranjbar R, Memariani M. Multilocus variable- number tandem-repeat analysis for genotyping of Shigella sonnei strains isolated from pediatric patients. Gastroenterol Hepatol Bed Bench 2015;8(3):225-32. [PMID] [PMCID]
12. Farajnia S, Azhari F, Alikhani MY, Hosseini MK, Peymani A, Sohrabi N. Prevalence of PER and VEB Type Extended Spectrum Betalactamases among Multidrug Resistant Acinetobacter baumannii Isolates in North-West of Iran. Iran J Basic Med Sci 2013;16(6):751-5. [PMID] [PMCID]
13. Ecker JA, Massire C, Hall TA, Ranken R, Pennella TT, Agasino Ivy C, et al. Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry. J Clin Microbiol 2006;44(8):2921-32. [DOI:10.1128/JCM.00619-06] [PMID] [PMCID]
14. Barbe V, Vallenet D, Fonknechten N, Kreimeyer A, Oztas S, Labarre L, et al. Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium. Nucleic Acids Res 2004;32(19):5766-79. [DOI:10.1093/nar/gkh910] [PMID] [PMCID]
15. Chang HC, Wei YF, Dijkshoorn L, Vaneechoutte M, Tang CT, Chang TC. Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region. J Clin Microbiol 2005;43(4):1632-9. [DOI:10.1128/JCM.43.4.1632-1639.2005] [PMID] [PMCID]
16. Dijkshoorn L, Aucken H, Gerner-Smidt P, Janssen P, Kaufmann ME, Garaizar J, et al. Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. J Clin Microbiol 1996;34(6):1519-25. [PMID] [PMCID]
17. Nemec A, Krizova L, Maixnerova M, van der Reijden TJ, Deschaght P, Passet V, et al. Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). Res Microbiol 2011;162(4):393-404. [DOI:10.1016/j.resmic.2011.02.006] [PMID]
18. Limansky AS, Zamboni MI, Guardati MC, Rossignol G, Campos E, Viale AM. Evaluation of phenotypic and genotypic markers for clinical strains of Acinetobacter baumannii. Medicina 2004;64(4):306-12.
19. Pantophlet R, Severin JA, Nemec A, Brade L, Dijkshoorn L, Brade H. Identification of Acinetobacter isolates from species belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with monoclonal antibodies specific for O Antigens of their lipopolysaccharides. Clin Diagn Lab Immunol 2002;9(1):60-5. [DOI:10.1128/CDLI.9.1.60-65.2002]
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