Modern Medical Laboratory Journal
Mod Med Lab J
Medical Sciences
http://modernmedlab.com
1
admin
2371-770X
2371-770X
10.30699/mmlj17
en
jalali
1396
10
1
gregorian
2018
1
1
1
1
online
1
fulltext
fa
Molecular Analysis of Shigella sonnei Strains Isolated from Hospitals of Tehran and Ilam Cities Using Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR)
روش های تشخیص آزمایشگاهی
Laboratory Methods
مقاله پژوهشی
Original Research Article
<p dir="ltr" style="text-align: justify"><em><strong>Background and Objective: </strong></em>Shigella species belong to the family Enterobacteriaceae, which cause dysentery with abdominal pain and tenesmus in human. Our country is one of the endemic and occasional epidemic areas of the disease. In the present study, Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR) technique that has high differentiation and specificity power compared to phenotypic markers, was used to investigate diversity and geographical distribution of colones as well as typing of Shigella strains.</p>
<p dir="ltr" style="text-align: justify"><em><strong>Materials and Methods: </strong></em>In this study, a total of 40 Shigella sonnei samples were isolated from stool of patients with diarrhea and divided into 5 groups. After identification and confirmation of the isolates by biochemical and serotyping methods, one colony of each isolate was cultured on LB medium and after DNA extraction, PCR was performed. After electrophoresis of the PCR product, gel images were saved in the computer for further analysis and typing of the isolates. </p>
<p dir="ltr" style="text-align: justify"><em><strong>Results: </strong></em>In this research, 40 isolates were examined using REP-PCR and a similarity matrix was constructed based on Dice's coefficient. According to this matrix, some isolates were completely similar (genetic similarity coefficient= 1) and some isolates showed the least similarity (genetic similarity coefficient= 0). The dendrogram was obtained using the UPGMA algorithm. The calculated Cophenetic correlation coefficient for this dendrogram was 0.91645.</p>
<p dir="ltr" style="text-align: justify"><strong><em>Conclusion</em>: </strong>From the results of the present study, it was concluded that we can type the Shigella sonnei strains using palindromic repetitive sequences. The extent of polymorphism indicates that REP-PCR technique is a useful method for genetic variation analysis in molecular typing of shigella sonnei strains. Although, some strains were completely similar, a high genetic variation was found among the studied population of Shigella sonnei. This level of variation is probably due to wide geographic distribution of this species in Iran.</p>
<p dir="ltr" style="text-align: justify"></p>
Shigella sonnei, REP-PCR, Molecular typing
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http://modernmedlab.com/browse.php?a_code=A-10-1-16&slc_lang=fa&sid=1
Shiva
Hosseini
10031947532846001545
10031947532846001545
No
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.
Abbas
Maleki
10031947532846001546
10031947532846001546
No
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.
Sobhan
Ghafourian
10031947532846001547
10031947532846001547
No
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.
Hossein
Kazemian
10031947532846001548
10031947532846001548
No
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.
Mohammad Hossein
Maleki
10031947532846001549
10031947532846001549
No
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.
Reza
Mohebi
10031947532846001550
10031947532846001550
No
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.
Noorkhoda
Sadeghifard
10031947532846001551
10031947532846001551
Yes
Clinical Microbiology Research Center, Ilam University of Medical Science, Ilam, Iran.