Modern Medical Laboratory Journal
Mod Med Lab J
Medical Sciences
http://modernmedlab.com
1
admin
2371-770X
2371-770X
10.30699/mmlj17
en
jalali
1401
4
1
gregorian
2022
7
1
5
2
online
1
fulltext
en
Knockdown of HSF1 sensitizes resistant prostate cancer cell line to chemotherapy
علوم پزشکی
Medical Sciences
مقاله پژوهشی
Original Research Article
<span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The treatment of prostate cancer patients usually starts with androgen ablation and followed by chemotherapy; however, in some cases the tumor develops resistant phenotype. Combination therapy is currently regarded as a cornerstone in cancer therapy to overcome the drug resistance. Herein, we investigated the combinatory effect of Docetaxel and Trastuzumab with a novel nanomedicine, BCc1. Also, we knocked down the expression of Heat shock factor-1, HSF1, in resistant Prostate Cancer cell line 3, PC3, using RNA interference, RNAi, to sensitize the cancer cells to the drug treatment. We observed down-regulation of Erb-B2 Receptor Tyrosine Kinase 3, ERBB3, B-Cell Leukemia/Lymphoma 2, BCL2, and Heat Shock Protein 90, HSP90, in HSF1 knockdown PC3 cells. Knockdown of HSF1 made PC3 cells more susceptible to Docetaxel treatment. Additionally, BCc1 nanomedicine was tested on prostate cancer cell line PC3 for the first time. It resulted in reduced metabolic activity in these cells. We propose that a combination of the gene therapy and the chemotherapy gives more favorable results in the treatment of refractory prostate cancer.</span></span>
Prostate Cancer, Combination therapy, Nanochelating Technology
47
55
http://modernmedlab.com/browse.php?a_code=A-10-874-1&slc_lang=en&sid=1
Roya
Yousefi
roya_ysf72@rocketmail.com
10031947532846002005
10031947532846002005
Yes
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Mahboubeh
Kabiri Renani
mkabiri@ut.ac.ir
10031947532846002006
10031947532846002006
No
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Fatemeh
Koohkan
f.kouhkan@yahoo.com
10031947532846002007
10031947532846002007
No
Stem Cell Technology Research Center, Tehran, Iran
Mahshid
Heidari
mahshid.heidari@gmail.com
10031947532846002008
10031947532846002008
No
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Sedigheh
Asad
asad@ut.ac.ir
10031947532846002009
10031947532846002009
No
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Symzar
Hosseinzadeh
symzar.hosseinzadeh@gmail.com
10031947532846002010
10031947532846002010
No
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohammad Hossein
Nazaran
nazaran@khazra.ir
10031947532846002011
10031947532846002011
No
4. Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran.