Modern Medical Laboratory Journal
Mod Med Lab J
Medical Sciences
http://modernmedlab.com
1
admin
2371-770X
2371-770X
10.30699/mmlj17
en
jalali
1400
8
1
gregorian
2021
11
1
4
2
online
1
fulltext
en
Iranian Scorpion (Odontobuthus bidentatus) crude venom change the redox potential of MCF-7 breast cancer cell line and induce apoptosis
علوم پزشکی
Medical Sciences
مقاله پژوهشی
Original Research Article
New natural substances obtained from scorpion venoms could be promising approaches for the treatment of cancers. Scorpion venom is a fully mixed compound that containing enzymes, non-enzymes, ions, and other organic compounds that induces apoptosis and necrosis in mammalian cells. In this study, the cytotoxicity effects, redox potential, and the ability of apoptosis induction of <em>Odontobuthus bidentatus</em> scorpion venom on MCF-7 cells were investigated. To do this, the MCF-7 cells were treated with the scorpion venom. MTT and neutral red assays was used to evaluate the cytotoxicity. Catalase, GSH and NO assays are used to determine the cells redox potential. Caspase-3 and cytochrome c release assays were exploited to investigate the apoptosis. The results of MTT and neutral red tests showed that <em>O. bidentatus</em> crude venom has cytotoxic effects on MCF-7 cells. Moreover, the results of catalase, GSH and NO assays showed that the crude venom could change the redox potential of MCF-7 cells, dose dependently which eventually lead to apoptosis. Also, the results of caspase-3 and the release of cytochrome c confirmed cell apoptosis. These results suggest that <em>O. bidentatus</em> venom is a suitable source of apoptosis-inducing compounds.
Odontobuthus bidentatus scorpion venom, MCF-7 cell line, Redox potential, Apoptosis induction
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http://modernmedlab.com/browse.php?a_code=A-10-73-7&slc_lang=en&sid=1
Hani
Keshavarz Alikhani
hani.keshavarz865@gmail.com
10031947532846001787
10031947532846001787
No
Department of Biology, Razi University, Kermanshah, Iran.
Jamil
Zargan
jazrgan@ihu.ac.ir
10031947532846001788
10031947532846001788
Yes
Department of Biology, Imam Hossein University, Tehran, Iran.
Ali
Bidmeshkipour
abidmeshki@razi.ac.ir
10031947532846001789
10031947532846001789
No
Department of Biology, Razi University, Kermanshah, Iran.
Ehsan
Zamani
meshkat110@chmail.ir
10031947532846001790
10031947532846001790
No
Department of Biology, Isfahan University, Isfahan, Iran.
Mohsen
Mosavi
smohsenmousavi71@gmail.com
10031947532846001791
10031947532846001791
No
Department of Biology, Imam Hossein University, Tehran, Iran.
Ahmad
Heidari
ag.heidari@gmail.com
10031947532846001792
10031947532846001792
No
Department of Production Engineering and Plant Genetic, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Ashkan
Hajinoormohammadi
kpahaji@ihu.ac.ir
10031947532846001793
10031947532846001793
No
Department of Biology, Imam Hossein University, Tehran, Iran.