TY - JOUR JF - Mod-Med-Lab-J JO - Mod Med Lab J VL - 1 IS - 1 PY - 2018 Y1 - 2018/1/01 TI - Assessment of the Relative Level of TNF-α Gene Expression in RAW264.7 Cells TT - N2 - Background and Objective: Recent studies have shown that not only the quantity, but also the quality of dietary fatty acids can affect lipid parameters. Elaidic acid is a major hydrogenated by-product of vegetable oil play a role in the development of cardiovascular disease, especially atherosclerosis, through changing lipid profile and increase in inflammation and inflammatory factors. On the other hand, the role of TNF-α is evident in inflammatory pathways and inflammation outcomes, which are involved in the initiation of atherosclerosis. Therefore, in the current study the effect of elaidic acid on TNF-α gene expression, was investigated. Method: RAW264.7 cells were treated with 100 and 200 μM of elaidic acid for 12 h. Then, expression of TNF-α gene was assessed after RNA extraction and cDNA synthesis. Results: TNF-α gene expression in RAW264.7 macrophage cell line showed no significant difference for both concentrations compared to the control. Conclusion: These results indicated that elaidic acid affect the development and acceleration of atherosclerosis in other ways than through TNF-α nuclear receptor. SP - 23 EP - 28 AU - Yeganeh Montakhab, Hossein AU - Dousti, Mohammad AU - Ahmadi Rezaie, Hossein Baba AD - Department of clinical biochemistry, Tehran University of Medical Science, Tehran, Iran KW - Cell culture KW - Elaidic acid KW - Atherosclerosis KW - Gene expression KW - Trans-fatty acid KW - TNF- α UR - http://modernmedlab.com/article-1-39-en.html DO - 10.30699/mmlj17-01-05 ER -