| Post date: 2021/04/26 |
Electrophoresis is a chromatography technique by which a mixture of charged molecules is separated according to size when placed in an electric field.
Electrophoresis is a general term that describes the migration and separation of charged particles (ions) under the influence of an electric field. An electrophoretic system consists of two electrodes of opposite charge (anode, cathode), connected by a conducting medium called an electrolyte. The separation effect on the ionic particles results from differences in their velocity (v), which is the product of the particle's mobility (m) and the field strength (E):
v=mE
The mobility (m) of an ionic particle is determined by particle size, shape, and charge, and the temperature during the separation, and is constant under defined electrophoretic conditions.
The ability of electrophoresis to separate charged species ranges from small inorganic or organic ions to charged biopolymers (like DNA or proteins), or even chromosomes, microorganisms, or whole cells.
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Electrophoresis is a general term that describes the migration and separation of charged particles (ions) under the influence of an electric field. An electrophoretic system consists of two electrodes of opposite charge (anode, cathode), connected by a conducting medium called an electrolyte. The separation effect on the ionic particles results from differences in their velocity (v), which is the product of the particle's mobility (m) and the field strength (E):
v=mE
The mobility (m) of an ionic particle is determined by particle size, shape, and charge, and the temperature during the separation, and is constant under defined electrophoretic conditions.
The ability of electrophoresis to separate charged species ranges from small inorganic or organic ions to charged biopolymers (like DNA or proteins), or even chromosomes, microorganisms, or whole cells.
Read more
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