MTT, Rapid Colorimetric Assay for Cellular Growth and Survival

 | Post date: 2021/03/15 | 
The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is the first widely accepted colorimetric assay to measure cell proliferation and viability. The use of MTT tetrazolium was first developed by Mossman in 1983. The mechanism of this assay based on the reduction of MTT, a yellow water-soluble tetrazolium dye, primarily by the mitochondrial dehydrogenases (nicotinamide adenine dinucleotide phosphate (NADPH)-dependent cellular oxidoreductase enzymes), to insoluble formazan crystals with purple color. Then, a solubilization solution should be added to dissolve the insoluble purple formazan into a colored solution. The absorbance of this colored solution can be quantified by measuring between 500 and 600 nm wavelength by a spectrophotometer.
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